Abstract

Grapevine Powdery Mildew is caused by the obligately biotrophic fungus Erysiphe (syn. Uncinula) necator. This plant disease leads to severe crop loss and subsequent economic burden for the wine and juice industry. While the life cycle of the fungus has been explained, the mechanisms underlying how the fungus surpasses the grape’s defenses are not fully understood. In order to identify suitable targets for gene silencing approaches, a better understanding of the Powdery Mildew transcriptome is necessary. De novo assembled contigs of E. necator G14 mRNA were compared to the published E. necator C-strain transcriptome to determine the areas of overlap between them. These were also aligned to the E. necator C-strain genome scaffold and annotated using both the NCBI non-redundant protein database as well as the Eggnog Orthologous group database. Evidence for novel transcription sites was found in the G14 transcriptome and these novel annotations were stored in a gff file for future use. The detection of previously unreported transcripts highlights the need for RNA sequencing approaches that can detect low copy number transcripts.

Library of Congress Subject Headings

Grape powdery mildew disease--Genetics; Genetic transcription

Publication Date

5-8-2018

Document Type

Thesis

Student Type

Graduate

Degree Name

Bioinformatics (MS)

Department, Program, or Center

Thomas H. Gosnell School of Life Sciences (COS)

Advisor

Michael Osier

Advisor/Committee Member

Lance Cadle-Davidson

Advisor/Committee Member

Gary R. Skuse

Campus

RIT – Main Campus

Plan Codes

BIOINFO-MS

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