Abstract

Five antibodies directed against digoxin were grown and characterized. Two separation techniques were tested to find the better method of separating bound the free fractions of ligand after competitive reactions with the antibodies. Charcoal separation was chosen. The antibodies were analyzed for affinity constant by the Scatchard Analysis. Cross reactivity data for two of the antibodies were collected. Four of the antibodies have satisfactory affinity constants for use in a digoxin assay. Cross reactivity studies showed varying amounts of cross reactivity with digoxin metabolites, but none with the steroids and lipid tested.

Library of Congress Subject Headings

Monoclonal antibodies--Testing; Digoxin--Measurement

Publication Date

1987

Document Type

Thesis

Student Type

Graduate

Degree Name

Chemistry (MS)

Department, Program, or Center

School of Chemistry and Materials Science (COS)

Advisor

Names Illegible

Comments

Note: imported from RIT’s Digital Media Library running on DSpace to RIT Scholar Works. Physical copy available through RIT's The Wallace Library at: QR186.85.A435 1987

Campus

RIT – Main Campus

Plan Codes

CHEM-MS

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