Microarray analysis of gene expression profiles of tetrahymena H2B ubiquitylation mutant strains

Author

Zhe Wang

Abstract

Microarray analyses were performed to compare the gene expression profiles of wild-type and mutant strains of Tetrahymena thermophila. These studies suggest that, in Tetrahymena, ubiquitylation of histone H2B is not required for H3K4 methylation. Even though the E2 ubiquitin-conjugating enzyme Ubc2 and the E3 ubiquitin ligase Bre1 are required for H2B ubiquitylation, these two enzymes affect the expression of a much broader spectrum of genes than H2B ubiquitylation, suggesting that Ubc2 and Bre1 are involved in pathways other than H2B ubiquitylation in Tetrahymena.

Library of Congress Subject Headings

Tetrahymena--Genetics; Gene expression; Mutation (Biology)

Publication Date

8-24-2010

Document Type

Thesis

Department, Program, or Center

Biomedical Sciences (CHST)

Advisor

Skuse, Gary

Advisor/Committee Member

Osier, Michael

Comments

Note: imported from RIT’s Digital Media Library running on DSpace to RIT Scholar Works. Physical copy available through RIT's The Wallace Library at: QL368.H87 W36 2010

Recommended Citation

Wang, Zhe, "Microarray analysis of gene expression profiles of tetrahymena H2B ubiquitylation mutant strains" (2010). Thesis. Rochester Institute of Technology. Accessed from
https://repository.rit.edu/theses/3702

Campus

RIT – Main Campus