This study investigated the relationship between the type of material and the concentration of IgA in human milk stored at two temperatures over time. Twelve breastfeeding mothers participated in the study with samples of milk obtained at locations of the participant's preference and transported to the laboratory in a sterile Snappies PP container stored in a cooler filled with ice. The sample was divided up and pipetted into 12 containers. IgA concentrations were determined by enzyme-linked immunosorbent assay at time zero, 9, 24, 48, 72 and 168 hours. Milk IgA concentration decreased in all containers, with the exception of polycarbonate from 72 hours to 168 hours in -20°C. In 4°C, the results of IgA concentrations showed volatility and did not indicate a stable trend. The data suggested that the concentration of milk IgA was more affected by temperature and the type of material may not have a significant impact on IgA. The data also indicated a maximum storage length of three days in refrigerators to maintain IgA levels.

Library of Congress Subject Headings

Breast milk--Packaging; Breast milk--Effect of temperature on; Immunoglobulin A--Effect of temperature on

Publication Date


Document Type


Student Type


Degree Name

Packaging Science(MS)

Department, Program, or Center

Packaging Science (CAST)


Thomas Frederick

Advisor/Committee Member

Deanna Jacobs

Advisor/Committee Member

Angela Foreman


RIT – Main Campus

Plan Codes