Fluorescent dyes tend to fatigue when exposed to short wavelength radiation. Since all work is not done at one pH level, it is necessary to know how a change in pH will effect the fluorescing characteristics of a dye. Of particular interest is the effect on fatigue as a function of time. The shift in spectral absorption with varying pH was established for fluorescein dye. Its increase in fluorescing intensity with increasing negative ion concentration was then measured. With increasing exposure time the pH's of 6.0, 8.0, and 10.0 gave indications of fatiguing while the pH's of 2.0 and 4.0 showed no change.

Library of Congress Subject Headings

Photomicrography; Photography--Scientific applications; Pierce, John R

Publication Date


Document Type

Senior Project

Student Type


Department, Program, or Center

School of Photographic Arts and Sciences (CIAS)


Not listed.


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