The virulent property of the Agrobacterium tumefaciens is associated with its tumor inducing (Ti) plasmid. Recent studies on the virulence of this bacterium has shown that genes located on its chromosome also contribute to this property. One such gene is thought to produce a protein that has properties similar to that of the recA protein of E. colt. This thesis outlines the techniques that were used to try and isolate the recA-like gene from the chromosome of the Agrobacterium tumefaciens. All the techniques used in this study are outlined in detail and an explanation given for the choice of each technique. For reasons not completely understood, we were unable to isolate the recA-like gene even though a gene bank was successfully constructed and appropriately sized fragments extracted. Attempts were made to explain some of the unexpected results and appropriate steps were proposed to further understand them.

Library of Congress Subject Headings

Cytogenetics; Gram-negative bacteria; DNA repair

Publication Date


Document Type


Department, Program, or Center

Biomedical Sciences (CHST)


Rothman, Robert

Advisor/Committee Member

Harrison, Howard

Advisor/Committee Member

Aumer, James


Note: imported from RIT’s Digital Media Library running on DSpace to RIT Scholar Works. Physical copy available through RIT's The Wallace Library at: QH434 .B377 1990


RIT – Main Campus